Affiliation:
1. *Department of Medical Microbiology and Hygiene, University of Ulm, Ulm, Germany; and
2. †Department of Microbiology, University of Rijeka, Rijeka, Croatia
Abstract
Abstract
The inflammatory response and influence of T cell depletion on the pathogenesis of an experimental Legionella infection were studied. A/J mice were infected with 106 CFU of Legionella pneumophila intratracheally. With this dose all infected animals survived the infection and bacteria were cleared from lung, spleen, liver, and kidney within 10 to 11 days, leaving no residual changes in the affected organs. Inflammatory cells were recruited into the lung on the second day of infection, reaching a maximum on the third day and filling out predominantly the interstitial areas. During the first 3 days after inoculation, mainly macrophages, B cells, NK cells, and large mononuclear cells of an unknown phenotype were attracted into the lung interstitium, whereas T lymphocytes infiltrated subsequently. During the early phase of infection, serum concentrations of IFN-γ, TNF-α, IL-1β, IL-4, and IL-6 but not IL-2 increased dramatically. The cytokine secretion decreased on the third day after infection although bacteria were still present in the lung or even disseminated in different organs. Successful clearance of bacteria from the lung was not observed before recruitment of T cells into the lung. In mice depleted of both CD4+ and CD8+ T cells, control of infection was impaired and lethality of infection increased. Depletion of either subset left residual antibacterial mechanisms, which, however, were not sufficient to clear the Legionella as rapidly as in undepleted mice.
Publisher
The American Association of Immunologists
Subject
Immunology,Immunology and Allergy
Reference28 articles.
1. Eisenstein, T. K., H. Friedman. 1985. Immunity to Legionella. S. M. Katz, ed. In Legionellosis Vol. II: 159 CRC Press, Boca Raton, FL.
2. Sheldon, P. A., R. R. Tight, E. D. Renner. 1985. Fatal Legionnaires’ disease coincident with initiation of immunosuppressive therapy. Arch. Intern. Med. 145: 1138
3. Cordonnier, C., J. P. Farcet, L. Desforges, C. Brun-Buisson, J. P. Vernant, M. Kuentz, R. Dournon. 1984. Legionnaires’ disease and hairy-cell leukemia. Arch. Intern. Med. 144: 2373
4. Friedman, A. P., S. M. Katz. 1981. The prevalence of serum antibodies to Legionella pneumophila in patients with chronic pulmonary disease. Am. Rev. Respir. Dis. 123: 238
5. Neumeister, B., M. Susa, B. Nowak, E. Straube, G. Ruckdeschel, J. Hacker, R. Marre. 1995. Enzyme immunoassay for detection of antibodies against isolated flagella of Legionella pneumophila. Eur. J. Clin. Microbiol. Infect. Dis. 14: 764
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