The Isolation in Tissue Culture, Chick Embryo and Suckling Mice of Filtrable Agents from Healthy Dairy Cattle

Abstract

Summary The isolation of eight filtrable agents from apparently healthy dairy cattle in cattle kidney tissue culture has been described. Evidence has been presented that they are viral in nature, serologically similar, fairly widespread in the cattle population and probably of little importance in human disease. Studies with the partition cell in the ultracentrifuge indicate that they are relatively small particles with a sedimentation constant of 150 to 200 Sved. Their host range includes monkey kidney tissue culture, chick embryos and 1-day-old suckling mice. The appearance of melanin-like granules in the amniotic fluid of pigmented Barred Rock chick embryos is of interest. The isolation of many new viral agents by the techniques outlined should be productive.