BRAIN-PROTECTIVE ACTIVITY OF A NOVEL COMPOUND – A HYDROXYPYRIDINE DERIVATIVE

Abstract

The cerebroprotective activity of a new compound 3-hydroxypyridine with ascorbic acid residue 3-EA was studied. The substance was synthesized at JSC All-Union Research Center of Biological Compounds Safety. Experiments were performed on 15 male Sprague-Dawley rats, which were unilaterally occluded under isoflurane anesthesia. Animals were randomly divided into 3 groups: sham-operated, control with ischemia, and experimental group, in which rats received 3-EA solution intravenously for 7 days after pathology modeling at a daily dose of 18 mg/kg. The rats of the first two groups received an equivalent volume of isotonic 0.9% sodium chloride solution. On days 1, 3 and 7, the neurological picture was recorded. On the 7th day, the volume of brain damage was assessed in the MTT test, and the degree of morphological disorders was determined in the prepared Nissl-stained sections. The results were evaluated by the methods of variation statistics. We found that the introduction of 3-EA at a dose of 18 mg/kg is accompanied by a decrease in the volume of ischemic damage to the brain tissue on the side of occlusion of the middle cerebral artery by an average of 17%, while when compared with animals in the control series, a reduction of more than two times is observed. the number of dead neurons against the background of a decrease in the depth of pathomorphological changes in the brain substance. With a dynamic assessment of the degree of neurological deficit (in the form of motor disorders and changes in sensitivity), there is an acceleration in the recovery of lost functions in the animals of the experimental group when compared with the control.