We prove that any region
Γ
\Gamma
in a homogeneous
n
n
-dimensional and locally compact separable metric space
X
X
, where
n
≥
2
n\geq 2
, cannot be irreducibly separated by a closed
(
n
−
1
)
(n-1)
-dimensional subset
C
C
with the following property:
C
C
is acyclic in dimension
n
−
1
n-1
and there is a point
b
∈
C
∩
Γ
b\in C\cap \Gamma
having a special local base
B
C
b
\mathcal B_C^b
in
C
C
such that the boundary of each
U
∈
B
C
b
U\in \mathcal B_C^b
is acyclic in dimension
n
−
2
n-2
. In case
X
X
is strongly locally homogeneous, it suffices to have a point
b
∈
C
∩
Γ
b\in C\cap \Gamma
with an ordinary base
B
C
b
\mathcal B_C^b
satisfying the above condition. The acyclicity means triviality of the corresponding Čech cohomology groups. This implies all known results concerning the separation of regions in homogeneous connected locally compact spaces.