In this work we characterize certain immersed closed hypersurfaces of some ambient manifolds via the second eigenvalue of the Jacobi operator. First, we characterize the Clifford torus as the surface which maximizes the second eigenvalue of the Jacobi operator among all closed immersed orientable surfaces of
S
3
\mathbb {S}^3
with genus bigger than zero. After, we characterize the slices of the warped product
I
×
h
S
n
I\times _h\mathbb {S}^n
, under a suitable hypothesis on the warping function
h
:
I
⊂
R
→
R
h:I\subset \mathbb {R}\to \mathbb {R}
, as the only hypersurfaces which saturate a certain integral inequality involving the second eigenvalue of the Jacobi operator. As a consequence, we obtain that if
Σ
\Sigma
is a closed immersed hypersurface of
R
×
S
n
\mathbb {R}\times \mathbb {S}^n
, then the second eigenvalue of the Jacobi operator of
Σ
\Sigma
satisfies
λ
2
≤
n
\lambda _2\le n
and the slices are the only hypersurfaces which satisfy
λ
2
=
n
\lambda _2=n
.