A Seifert matrix is a square integral matrix
V
V
satisfying
det
(
V
−
V
T
)
=
±
1.
\begin{equation*}\det (V - V^T) =\pm 1. \end{equation*}
To such a matrix and unit complex number
ω
\omega
there corresponds a signature,
σ
ω
(
V
)
=
sign
(
(
1
−
ω
)
V
+
(
1
−
ω
¯
)
V
T
)
.
\begin{equation*}\sigma _\omega (V) = \mbox {sign}( (1 - \omega )V + (1 - \bar {\omega })V^T). \end{equation*}
Let
S
S
denote the set of unit complex numbers with positive imaginary part. We show that
{
σ
ω
}
ω
∈
S
\{\sigma _\omega \}_ { \omega \in S }
is linearly independent, viewed as a set of functions on the set of all Seifert matrices. If
V
V
is metabolic, then
σ
ω
(
V
)
=
0
\sigma _\omega (V) = 0
unless
ω
\omega
is a root of the Alexander polynomial,
Δ
V
(
t
)
=
det
(
V
−
t
V
T
)
\Delta _V(t) = \det (V - tV^T)
. Let
A
A
denote the set of all unit roots of all Alexander polynomials with positive imaginary part. We show that
{
σ
ω
}
ω
∈
A
\{\sigma _\omega \}_ { \omega \in A }
is linearly independent when viewed as a set of functions on the set of all metabolic Seifert matrices. To each knot
K
⊂
S
3
K \subset S^3
one can associate a Seifert matrix
V
K
V_K
, and
σ
ω
(
V
K
)
\sigma _\omega (V_K)
induces a knot invariant. Topological applications of our results include a proof that the set of functions
{
σ
ω
}
ω
∈
S
\{\sigma _\omega \}_ { \omega \in S }
is linearly independent on the set of all knots and that the set of two–sided averaged signature functions,
{
σ
ω
∗
}
ω
∈
S
\{\sigma ^*_\omega \}_ { \omega \in S }
, forms a linearly independent set of homomorphisms on the knot concordance group. Also, if
ν
∈
S
\nu \in S
is the root of some Alexander polynomial, then there is a slice knot
K
K
whose signature function
σ
ω
(
K
)
\sigma _\omega (K)
is nontrivial only at
ω
=
ν
\omega = \nu
and
ω
=
ν
¯
\omega = \overline {\nu }
. We demonstrate that the results extend to the higher-dimensional setting.