Development of continuous flow injection analysis method for determination of oxymetazoline and vancomycin hydrochloride in pharmaceutical preparations

Abstract

ABSTRACT. Continuous flow injection (CFI)-spectrophotometric method has been developed for oxymetazoline (OXY) and vancomycin hydrochloride (VAN) determination in pure and dosage forms. Sulfadimidine drug was used as a safe chromogenic reagent by diazotization coupling with studied drugs to produce sensitive azo dyes with maximal wavelengths of 498 and 441 nm, respectively, for OXY and VAN drugs. The effect of various chemical and physical conditions on the signal response has been studied. Under optimum conditions, Beer’s law was linear over the concentration range of 5–200 and 6–200 µg/mL with limits of detection of 2.19 and 1.79 µg/mL for OXY and VAN, respectively. The CFI systems gave a sample throughput of more than 120 samples per hour for 150 μL injection volume for both drugs. The proposed methods were used for the estimation of microgram quantities of both drugs in their different dosage forms with great effectiveness. The method's validity and applicability were thoroughly investigated and the recoveries values were compared with those of standard pharmacopeia methods. The specified approaches were used without the excipients interfering in pharmaceutical applications.   KEY WORDS: Oxymetazoline hydrochloride, Vancomycin Hydrochloride, Flow injection, spectrophotometry, Pharmaceutical formulations   Bull. Chem. Soc. Ethiop. 2022, 36(2), 303-313.                                                               DOI: https://dx.doi.org/10.4314/bcse.v36i2.5