Ultrasound-assisted dispersive liquid-liquid microextraction approach for preconcentration of acyclovir as antiviral drug in dosage forms prior to spectrophotometric determination

Abstract

ABSTRACT. For acyclovir (ACV) determination in bulk and dosage forms, quick, sensitive, straightforward, and eco-friendly ultrasound-assisted dispersive liquid-liquid microextraction (UA-DLLME)-based spectrophotometric method has been created and validated. ACV with 1,2-naphthoquine-4-sulfonate (NQS) react in alkaline medium to produce a yellow-colored product, which is the basis of the newly created method. Investigation and optimization were done on the crucial experimental variables influencing ACV's extraction effectiveness. At λmax = 495 nm, the minuscule organic droplets were detected. The linearity was present from 0.1 to 3.0 μg/mL under ideal circumstances, with a linear correlation coefficient of 0.9995. The detection limit was 0.03 μg/mL and quantification limit was 0.1 μg/mL. 22.50 was the enrichment factor. Relative standard deviation (RSD%) as accuracy at 2.0 μg/mL of ACV was 1.0%, with good recovery (99.30%). The developed UA-DLLME method was effectively used to determine the ACV in dosage forms, and the validation was evaluated. Results from the suggested technique for dosage forms and pure ACV were in excellent agreement with the results from the official method.   KEY WORDS: Ultrasound-assissted, Dispersive liquid–liquid microextraction, Acyclovir, Spectrophotometry, Dosage forms Bull. Chem. Soc. Ethiop. 2023, 37(5), 1081-1092.                                                        DOI: https://dx.doi.org/10.4314/bcse.v37i5.2