SPAG9 promotes the migration and invasion of melanoma cells by regulating the MAPK pathway

Author:

Liu Yongjun,Zhu Xuefeng,You Xiaoen,Kang Runfang

Abstract

Purpose: To determine the regulatory role of sperm-associated antigen 9 (SPAG9) in melanoma cell growth. Methods: Immunohistochemical analysis was performed to investigate SPAG9 expression in melanoma tissues. Western blot was used to evaluate SPAG9 expression in melanoma tissues and cells. Melanoma cells were transfected with an siRNA targeting SPAG9 or a SPAG9 overexpression vector. Cell migration and invasion were examined by wound healing and transwell assays. The effects of SPAG9 on the epithelial-mesenchymal transition and mitogen-activated protein kinase (MAPK) pathway in melanoma cells were assessed by western blot. Results: SPAG9 expression was enhanced in melanoma tissues and cells. SiRNA-mediated silencing of SPAG9 repressed melanoma cell migration and invasion, and SPAG9 overexpression contributed to melanoma cell metastasis. In melanoma cells transfected with siRNA-SPAG9, E-cadherin expression decreased while vimentin and matrix metalloproteinase (MMP)-2/9 expression increased. However, ectopic expression of SPAG9 reversed this expression of E-cadherin, vimentin, and MMP-2/9. Silencing of SPAG9 decreased the phosphorylation of MAPKs, including p-p38, p-ERK and p-JNK, in melanoma cells. SPAG9 overexpression upregulated phosphorylation of MAPKs. Conclusion: SPAG9 promotes the migration and invasion of melanoma cells by activating the MAPK pathway.

Publisher

African Journals Online (AJOL)

Subject

Pharmacology (medical),Pharmaceutical Science

Cited by 1 articles. 订阅此论文施引文献 订阅此论文施引文献,注册后可以免费订阅5篇论文的施引文献,订阅后可以查看论文全部施引文献

1. MiR-22 alleviates the proliferation and metastasis of melanoma by targeting FASN;Tropical Journal of Pharmaceutical Research;2022-11-03

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