Evidence of anti-inflammatory and anti-ulcer properties of aerial parts of Centaurea tougourensis Boiss. and Reut.

Abstract

Purpose: To determine the anti-inflammatory and anti-ulcer properties of the aerial parts of Centaurea tougourensis Boiss. & Reut. Methods: The effects of n-butanol (n-BuOH) extract of the aerial part of Centaurea tougourensis on carrageenan-induced paw edema and ethanol-induced gastric mucosal damage were determined at 2 doses (200 and 400 mg/kg, po) in a mouse model. For each test, the animals were randomly divided into negative and positive control groups, as well as extract-treated groups. The mice were observed for any sign of inflammation for a period of 24h. Results: Reduction of paw edema by C. tougourensis extract was highly significant (p < 0.001) at a dose of 400 mg/kg 24 h after carrageenan injection, with 55.26 % inhibition, followed very closely by 53.15 % inhibition at the dose of 200 mg/kg; indomethacin group showed an inhibition of 60 %. Histological examination supported the inhibition results. A significant reduction in inflammation by the extract at a dose of 400 mg/kg was also observed. No sign of ulcer was observed with C. tougourensis at the two doses (200 and 400 mg/kg). The total polyphenol content of the n-BuOH extract was 85.44 цg gallic acid equivalent/mg of extract. Tannins were the most abundant fraction (51.87 цg tannic acid equivalent/mg of extract), followed by flavonoids (25.55 цg quercetin equivalent/mg of extract). Conclusion: The results indicate that C. tougourensis may have potential beneficial effects in the treatment of diseases associated with inflammation and pain, besides its protective effect on the gastrointestinal tract.