HOXC10 promotes nasopharyngeal carcinoma cell proliferation and migration by regulating PI3K/AKT pathway

Abstract

Purpose: To evaluate the role of homeobox C10 (HOXC10) in nasopharyngeal carcinoma (NPC). Methods: Cell Counting Kit-8 (CCK-8) and colony formation assays were conducted to determine NPC cell proliferation. Cell migration and invasion were assessed using a Transwell assay, while western blot was used to investigate the mechanism of action involved in HOXC10- mediated NPC. Results: HOXC10 levels were significantly elevated in NPC cells (p < 0.001). Over-expression of HOXC10 significantly increased NPC cell viability (p < 0.05) and proliferation. However, silencing HOXC10 reduced NPC cell proliferation. HOXC10 knockdown suppressed NPC cell migration and invasion. NPC expression of phosphorylated phosphoinositide-3-kinase (PI3K) and protein kinase B (AKT) proteins were up-regulated after HOXC10 over-expression but were down-regulated upon silencing HOXC10 (p < 0.05). Conclusion: HOXC10 knockdown reduces NPC cell proliferation and metastasis by inactivating PI3K/AKT pathway, and therefore, can potentially be developed for the treatment of nasopharyngeal carcinoma.