Lidocaine, an anesthetic drug, protects Neuro2A cells against cadmium toxicity
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Published:2021-05-25
Issue:5
Volume:18
Page:1033-1039
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ISSN:1596-9827
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Container-title:Tropical Journal of Pharmaceutical Research
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language:
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Short-container-title:Trop. J. Pharm Res
Author:
Chen Peng,Zhang Wenyu,Li Xuefeng,Li Longyun
Abstract
Purpose: To investigate the neuroprotective effect of lidocaine in Neuro2A cells
Methods: Differentiated N2a cells were used in this study. Cell viability and neuroprotection were assessed using dimethylthiazol-2-yl)-2,5-diphenyltetrazolium bromide (MTT) and trypan blue assays, while Bax/Bcl-2 expression was assayed by western blotting. Mitochondrial membrane potential, reactive oxygen species and calcium levels were measured using flow cytometry.
Results: Lidocaine protected differentiated N2a cells against cadmium-induced toxicity, and also attenuated cadmium toxicity-induced changes in mitochondrial membrane potential (MMP), reactive oxygen species (ROS) and calcium (Ca2+) levels. Furthermore, Bax/Bcl-2 ratio, which was disrupted by cadmium, and cadmium-induced apoptosis, were reversed by lidocaine.
Conclusion: Lidocaine protects differentiated N2a cells against cadmium-induced toxicity by reversing apoptosis. Thus, lidocaine is a potential neuroprotective agent.
Publisher
African Journals Online (AJOL)
Subject
Pharmacology (medical),Pharmaceutical Science