Antidiabetic potential of Nigella sativa L seed oil in alloxaninduced diabetic rabbits

Abstract

Purpose: To evaluate the antidiabetic, hypolipidemic and antioxidant potential of seed oil of Nigella sativa L (NSO). Methods: Nigella sativa seed oil (NSO) was extracted with Soxhlet apparatus using petroleum ether, and was given orally at a dose of 2.5 ml/kg body weight to alloxan-induced diabetic rabbits daily for 24 days. Biochemical parameters including total cholesterol (TC), triglycerides (TGs), low density lipoprotein cholesterol (LDL), very low lipoprotein cholesterol (VLDL), high density lipoprotein (HDL) and plasma glucose were determined in the treatment and control groups. Furthermore, bilirubin, vitamin C, catalase and mean body weight were assessed. Results: NSO treatment significantly lowered serum blood glucose levels and lipid contents, but increased the mean body weight, HDL-C and vitamin C levels of diabetic rabbits (p < 0.001). Moreover, NSO significantly decreased catalase activity, TC, TGs, LDL-C and VLDL-C levels, but normalized bilirubin levels in diabetic rabbits. Conclusion: These results indicate that NSO possesses significant antidiabetic potential. Thus, it may be useful as an adjunct with antidiabetic medication but further studies are required to ascertain this. Keywords: Black cumin, Diabetes, Hypoglycemic, Hypolipidemic, Antioxidants