Effect of capecitabine and melatonin on HER2+ (SK-BR-3) and HER2- (MCF7) human breast cancer cell lines
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Published:2023-08-19
Issue:7
Volume:22
Page:1387-1393
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ISSN:1596-9827
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Container-title:Tropical Journal of Pharmaceutical Research
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language:
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Short-container-title:Trop. J. Pharm Res
Author:
Shayan Sepideh,Osgoei Laya Takbiri,Jouni Fatemeh Javani
Abstract
Purpose: To determine the effect of melatonin in combination with capecitabine on the proliferation and induction of apoptosis in MCF-7 and SK-BR-3 breast cancer cell lines.Methods: The MTT assay (3- (4,5-dimethylthiazol-2-yl) -2,5-diphenyltetrazolium bromide) was performed to investigate the effect of capecitabine and in combination with melatonin on cell lines and to determine the half-maximal concentration (IC50) and combination index. Expression of apoptotic markers, Bax, Bcl-2, and caspase-3 were measured by polymerase chain reaction (PCR) after treatment.Results: The IC50 of melatonin and capecitabine in MCF-7 and SK-BR-3 cell lines were 4.52 mM and 619.36 μg/mL, and 5.1 mM and 679.51 μg/mL, respectively. The combined use of melatonin and capecitabine significantly reduced IC50. Also, combination index (CI) values were < 1, indicating that the combination of capecitabine and melatonin has a synergistic effect. The results of gene expression also showed enhancement in the Bax/Bcl-2 ratio in melatonin-capecitabine combination compared with capecitabine alone in both cell lines.Conclusion: Melatonin-capecitabine-induced cell death is controlled by caspase-3 and Bax/Bcl-2-dependent apoptosis. The combination of melatonin and capecitabine has a synergistic effect on both HER2+ and HER2- cells.
Publisher
African Journals Online (AJOL)
Subject
Pharmacology (medical),Pharmaceutical Science
Cited by
1 articles.
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