Anti sickling effect of Senna alexandrina, Aerva javanica , and Ficus palmata extracts on sickle cell disorder
-
Published:2023-04-19
Issue:3
Volume:22
Page:571-577
-
ISSN:1596-9827
-
Container-title:Tropical Journal of Pharmaceutical Research
-
language:
-
Short-container-title:Trop. J. Pharm Res
Author:
Yousef Zahraa M Al,Alaswad Elaf A,ElAssouli M‑Zaki M,Nori Dunya A,Filimban Faten Z,Choudhry Hani,Alam Mohammad Z,Sadoun Hadeel Al,Helmi Nawal MW
Abstract
Purpose: To investigate the anti-sickling activity of Senna alexandrina, Aerva javanica, and Ficus palmate extracts. Methods: The leaves were extracted with water and methanol. The anti-sickling effect of extracts was determined using blood from 20 sickle cell disease (SCD) patients by mixing it with 500 and 1000 μg/mL of extract. The treated blood samples were then examined under a light microscope. The osmotic fragility of erythrocytes was determined using 4 mL (0 – 0.9 %) buffered saline mixed with 1 mL extract (500 μg/mL) and 0.05 mL sickled red blood cells (RBCs). Results: Aerva javanica extract significantly (p < 0.0001) reduced the percentage of sickle cells compared to control. The sickle-shaped cells were 29.47 % when treated with 125 μg/mL concentration of methanol extract of A. javanica that was further decreased to 0.35 % on increasing the concentration to 500 μg/mL. A similar trend was also observed with extracts of F. palmata. Extracts had protective effect on erythrocyte osmotic fragility. Results of comet assay suggested that extracts did not damage DNA. Conclusion: These plant extracts have promising anti-sickling activity and protect erythrocyte osmotic fragility. However, in vivo studies are required to validate these results and for further development of the extracts for therapeutic use.
Publisher
African Journals Online (AJOL)
Subject
Pharmacology (medical),Pharmaceutical Science