Depletion of circ_0001313 suppresses the carcinogenesis of laryngeal carcinoma through miR 512 5p/HMGA1 pathway

Abstract

Purpose: To probe the influence of circ_0001313 on the occurrence and development of laryngeal carcinoma and the potential molecular mechanism. Methods: 27 cases of laryngeal carcinoma attending health facility from January 2017 to September 2020 were obtained. The contents of circ_0001313, miR-512-5p and HMGA1 in the tissues were detected. Laryngeal carcinoma TU686 cells were randomly divided into different group via assigned transfection, and cell growth and mobility were evaluated. The investigation of the relationship was then determined. Results: Laryngeal carcinoma tissues and TU686 cell lines showed high expressed circ_0001313 and HMGA1, as well as low expressed miR-512-5p. Circ_0001313 deficiency or miR-512-5p overexpression weakened the survival, migration and invasiveness of TU686 cells. Circ_0001313 sponged miR-512-5p, which bound to HMGA1. In TU686 cells, the content of miR-512-5p was markedly increased with circ_0001313 depletion, and HMGA1 protein level was decreased with miR-512-5p upregulation. The deficiency of miR-512-5p weakened the influence of circ_0001313 depletion on the growth and mobility of TU686 cells. Conclusion: Interference of circ_0001313 could impair the migration, proliferation, and invasiveness of laryngeal carcinoma cells and promote cell apoptosis by regulating miR-512-5p/HMGA1 axis.