Purification of Stearidonic Acid (18:4(n-3)) and Hexadecatetraenoic Acid (16:4(n-3)) from Algal Fatty Acid with Lipase and Medium Pressure Liquid Chromatography

Author:

ISHIHARA Kenji1,MURATA Masakazu1,KANENIWA Masaki1,SAITO Hiroaki1,KOMATSU Wataru2,SHINOHARA Kazuki3

Affiliation:

1. Marine Biochemistry Division, National Research Institute of Fisheries Science

2. Department of Life Science, Toita Women's College

3. National Food Research Institute

Publisher

Oxford University Press (OUP)

Subject

Organic Chemistry,Molecular Biology,Applied Microbiology and Biotechnology,General Medicine,Biochemistry,Analytical Chemistry,Biotechnology

Reference13 articles.

1. 1) Food and Agriculture Organization of the United Nations, FAO Yearbook of Fishery Statistics-Catches and landings, 76, 450-454 (1993).

2. 2) Takagi, T., Asahi, M., and Itabashi, Y., Fatty acid composition of twelve algae from Japanese waters. Yukagaku, 34, 1008-1012 (1985).

3. 3) Ishihara, K., Murata, M., Kaneniwa, M., Saito, H., Shinohara, K., and Maeda-Yamamoto, M., Inhibition of icosanoid production in MC/9 mouse mast cells by n-3 polyunsaturated fatty acids isolated from edible marine algae. Biosci. Biotechnol. Biochem., 62, 1412-1415 (1998).

4. 4) Huang, Y. S., Smith, R. S., Redden, P. R., Cantrill, R. C., and Horrobin, D. F., Modification of liver fatty acid metabolism in mice by n-3 and n-6 delta 6-desaturase substrates and products. Biochim. Biophys. Acta, 1082, 319-327 (1991).

5. 5) Yamazaki, K., Fujikawa, M., Hamazaki, T., Yano, S., and Shono, T., Comparison of the conversion rates of alpha-linolenic acid (18:3(n-3)) and stearidonic acid (18:4(n-3)) to longer polyunsaturated fatty acids in rats. Biochem. Biophys. Acta, 1123, 18-26 (1992).

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