Abstract
Goal of the research is to consider the main concepts used in the probit-analysis method, the features of its application to determine the toxicity of pesticides, in particular insecticides, acaricides and fungicides.
Methods. To determine the toxicity of pesticides, the method of probit-analysis was used. It consists in determining the dose of a substance necessary to achieve a certain level of mortality of test organisms, based on the transformation of the S-shaped «dose-effect» curve into a straight line.
Results. The article describes in detail the main stages of probit-analysis: selection of biological material; determination of the pesticide dose range to be used and preparation of working solutions; processing of biological material; assessing the mortality of the studied objects; processing of the obtained results. The peculiarities of its use in determining the toxicity of insecticides and fungicides of different chemical groups and with different mechanisms of action are shown. As an example, the application of the method for determining the toxicity of insecto-acaricide Vertimek 018 EC against the spider mite (Tetranychus urticae Koch.) is given.
Conclusion. The results of the research show the effectiveness of the probit-analysis method in determining the toxicity of pesticides, the possibility of its application for assessing the sensitivity of pests and pathogens to pesticides, and monitoring of their resistance.
Publisher
Institute of Plant Protection of NAAS
Subject
General Earth and Planetary Sciences,General Environmental Science
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