Antibacterial Activity of Some Nano Particles on Antibiotic-resistant Bacterial Pathogens from the Air of Operation Theatre

Abstract

The current research work was carried out to find the antibacterial activity of some nano particles against bacterial pathogens isolated from the air of operation theatre of Mayo hospital, Lahore, Pakistan. Three pathogenic bacterial strains were isolated, namely A1, A2, A3. Molecular characterisation, optimum growth conditions and antibiotic resistance of bacterial isolates were checked. The antibiotics used in this study were Amoxycillin, Cefepime and Ampicillin. Nano particles were used in methanolic solutions (mg/ml). Nano particles included ferric oxide, Zinc oxide and Silver Oxide. Results showed A3 was resistant to all antibiotics. Other strains showed sensitivity and resistance to these three antibiotics. All nano particles showed antibacterial activity against pathogenic bacterial isolates. Maximum zone of inhibition of 1 cm was formed when used Ferric oxide against the A1 bacterial pathogen. Optimum temperature was 37°C while the optimum pH was 7.  These bacterial pathogens were identified by ribotyping as Staphylococcus aureus (A1), Pseudomonas aeruginosa (A2) and Streptococcus pyogenes (A3).