The Impact of Zinc Oxide Nanoparticle on LH, FSH, and Testosterone Hormones in Mature Male NMRI Rats

Abstract

Nanoparticles are widely applied in all aspects of modern life because of their unique features such as small size and high surface area. Several types of research have been carried out to discover the feasible detrimental impacts of Nano-particles on human reproduction. The purpose of this study was to examine the impact of zinc oxide nanoparticles in mature male rats through examining LH, FSH, and testosterone sex hormones. Therefore, 30 Naked Mole-Rat Initiative (NMRI) rats were divided into 5 groups. Different doses of zinc oxide nanoparticles (250, 500 and 700 mg.kg-1) were intra peritoneally injected to animals only once. Then, the serum level of luteinizing hormone (LH), Follicle Stimulating Hormone (FSH), and testosterone hormones were measured using Enzyme-Linked Immunosorbent Assay (ELISA) method after 21 days. The results were analyzed by ANOVA and Tukey tests. The results indicated that zinc oxide nanoparticles doses caused a significant increase in FSH and testosterone level of blood (Respectively) in 250 and 700mg.kg-1 in comparison with the control group. Moreover, this research illustrated that zinc oxide nanoparticle can cause a dose-related increase in Testosterone and FSH hormones levels while causing no significant change in LH hormone level.