Author:
Vemuri Praveen Kumar,Varakala Nikhil Reddy,Dhakate Divyanshu,Ravavarapu Tripura,Dumpala Faina Philberta,Muddana Susmita Sri,Bommepalli Haritha,Modiboyana Spurthi
Abstract
Background: Regardless of the broad explore in the territory of glycobiology concerning structure and capacity of glycans, lectins and glycosylation forms, numerous viewpoints are still left unexplored.
Aim: In this study, we analyzed the effect of shuttle vector on the secretion of human galectin recombinant protein.
Methods: The galectin was expressed in E. coli BL21 by growing the bacterial culture in SOC medium and purified by nickel-based affinity chromatography due to its His-tag.
Results: After cell lysis the protein was identified as a single 29 KDa band by 12% SDS-PAGE. Conclusion: Characterization studies clearly revealed that the purified protein was indeed galectin 3.
Publisher
Sciencedomain International
Cited by
2 articles.
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