Spectrophotometric Assessment of Benzene 1,3,5-Triol in Pure Forms and in Bulk Sample by Diazotiization Coupling Reaction

Abstract

The method was published for determining microgram quantities of benzene 1,3,5-triol (phloroglucinol) in aqueous solution and some bulk samples using an easy, quick, and effective spectrophotometric method. The method uses a diazotization as well as coupling process in basic medium to produce a bright yellow water-soluble dye from Phloroglucinol and diazotized 4-Methoxy aniline That is stable and also has a maximum absorption wavelength of 420 nm. With a molar absorptivity of 1.5989x 104l mol-1 cm-1 as well as index of Sand ell sensitivity 0.001 g.cm- 2, Beer's law is followed across a concentration range of (2-40) g.ml-1 of phloroglucinol.The ideal conditions for all color development are detailed, and even the suggested procedures for determining phloroglucinol in aqueous medium as well as some bulk sample  have been successfully employed.