Wound Healing Potential of Rhodomyrtus tomentosa and its Bioactive Compounds- Rhodomyrtone

Abstract

Aims: The present work was aimed to study the phytochemical composition of a crude ethanolic extract of Rhodomyrtus tomentosa [SERT], and the presence of rhodomyrtone and SERT's in vitro wound healing activity. Introduction: Rhodomyrtus tomentosa is native plant to southern and southeastern Asia, India, east to southern China, Taiwan, Philippines, and south to Malaysia. In the traditional Vietnamese, Chinese and Malaysia, all its part, including leaves, roots, buds, and fruits have been used. A need for a new source of wound healing agent is the call for the investigation of the potential of R. tomentosa as the source of health-promoting agent, specifically as a natural wound healing agent. Methodology: SERT was screen for its phytochemicals and the detection of rhodomyrtone using liquid chromatography–mass spectrometry, /Quadrupole time-of-flight [LC-MS/QTOF] analysis. Cell viability, cell proliferation, and migration assay were performed to examine the SERT effect's in vitro wound healing activity on human fibroblast cells [CRL-2522]. Results: The phytochemical study showed the presence of saponins, flavonoids, tannins and steroid in the crude ethanolic extract. The LC-MS analysis of crude ethanolic extract of SERT showed presents of rhodomyrtone which is one of the major compounds in the extract. SERT exhibit proliferative and migratory rate in human fibroblast cells [CRL 2522] in dose-dependent manner, which supports wound healing process. Its bioactive compounds presented wound healing activities at 0.325 up to 2.5 µg/mL. Conclusion: Both SERT and rhodomyrtone portrayed in vitro wound healing activities. Further studies to elucidate the mechanism of action of SERT and rhodomyrtone is recommended.