Micellar liquid chromatography in doping control

Abstract

The issue of doping control in sport involves the development of reliable analytical procedures and efficient strategies to process a large number of samples in a short period of time. Reversed-phase LC techniques with aqueous–organic mobile phases and MS or diode-array detection yield satisfactory results for the identification of prohibited substances in sport. However, time-consuming sample pretreatment steps are required, which reduces sample throughput. Micellar LC (MLC) that uses hybrid mobile phases of surfactant above its critical micellar concentration and organic solvent has been revealed as an interesting alternative. The surfactant sodium dodecyl sulfate solubilizes the protein components of urine, serum and plasma, which permits their direct injection into the chromatographic system. Only dilution and filtering of the samples may be required. Most MLC analyses are performed in isocratic mode, with short retention times and good selectivity. The sensitivity of MLC allows the detection of a variety of doping substances at least 24–48 h after being administered.