Evaluation of dried blood spot sampling following cassette dosing in drug discovery-Reference-Cited by-同舟云学术

Evaluation of dried blood spot sampling following cassette dosing in drug discovery

Published:2011-10 Issue:20 Volume:3 Page:2291-2302
ISSN:1757-6180
Container-title:Bioanalysis
language:en
Short-container-title:Bioanalysis

Author:

Liang Xiaorong¹,Yang Lulu¹,Berezhkovskiy Leonid¹,Liederer Bianca¹,Nguyen Trung²,Jia Wei³,Schweiger Michelle²,Dean Brian¹,Deng Yuzhong

Affiliation:

1. Drug Metabolism & Pharmacokinetics, Genentech Inc., South San Francisco, CA 94080, USA

2. Pharmaceutics, Genentech Inc., South San Francisco, CA 94080, USA

3. Department of Safety Assessment, Genentech Inc., South San Francisco, CA 94080, USA

Abstract

Background: Dried blood spot (DBS) sampling has received growing interest mainly in regulatory preclinical and clinical studies while not routinely used in exploratory discovery pharmacokinetic screening. An intravenous bolus cassette dose of six compounds in rats followed by hemolyzed blood sample (HBS) and DBS sampling was evaluated in this study. Results: A sensitive liquid chromatography tandem mass spectrometry method was developed and qualified for the simultaneous determination of six compounds in rat whole blood using DBS or HBS techniques. The concentrations obtained from DBS samples matched well with those from HBS for each individual compound. Conclusion: This study demonstrated the applicability of DBS sampling for cassette dosing in discovery pharmacokinetics screening.

Publisher

Future Science Ltd

Subject

Medical Laboratory Technology,Clinical Biochemistry,General Pharmacology, Toxicology and Pharmaceutics,General Medicine,Analytical Chemistry

Link

http://www.future-science.com/doi/pdf/10.4155/bio.11.205

Reference14 articles.

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2. Dried blood spot sampling in combination with LC-MS/MS for quantitative analysis of small molecules

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