Fluoroplast-polyaniline-coated adsorbent for one-step isolation of DNA for PCR detection of viral hepatitides (HBV and TTV)

Abstract

Aims: To demonstrate the effectiveness of application of the adsorbent successively modified with nano-layers of fluoroplast and polyaniline for one-step isolation of DNA of hepatitis B virus and transfusion-transmitted virus from human serum. Materials & Methods: The technique is based on the application of the spin-cartridges containing porous adsorbent for one-step viral DNA isolation from serum followed by polymerase chain reaction. Results: The developed adsorbent was shown to be effective for one-step isolation of viral DNA from serum samples for polymerase chain reaction diagnostics. Conclusion: The effectiveness of the developed adsorbent application for isolation of viral DNA from serum for polymerase chain reaction diagnostics was confirmed in comparison with standard methods. Thus, the facile sample preparation method of viral DNA isolation was elaborated.