Definitive profiling of plasma bile acids as potential biomarkers for human liver diseases using UPLC–HRMS

Author:

Shi Yao1,Gao Yang1,Parys Michael Van1,Miller Dennis1,Milanowski Dennis1,Dean Brian2,Liang Xiaorong2

Affiliation:

1. Bioanalytical Department, Covance Laboratories, Inc., 3301 Kinsman Blvd, Madison, WI 53704, USA

2. Drug Metabolism & Pharmacokinetics, Genentech, 1 DNA way, South San Francisco, CA 94080, USA

Abstract

Aim: Investigation of bile acids (BAs) as biomarkers for liver and kidney diseases has gained momentum recently to fulfill the needs in drug development and clinical practice, but a thorough and rapid profiling of BAs in human plasma has been hindered by the large interindividual variability and lack of selective methods. Results: A selective and efficient UPLC-high resolution mass spectrometry method was developed and fully validated for the definitive profiling of 26 BAs in human plasma with a curve rage of 1–1000 ng/ml and a runtime of 7.2 min. Conclusion: Four BA combinations with good sensitivity and specificity show potential biomarker applications for liver injury and diseases.

Publisher

Future Science Ltd

Subject

Medical Laboratory Technology,Clinical Biochemistry,General Pharmacology, Toxicology and Pharmaceutics,General Medicine,Analytical Chemistry

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