Electromembrane extraction of substances with weakly basic properties: a fundamental study with benzodiazepines-Reference-Cited by-同舟云学术

Electromembrane extraction of substances with weakly basic properties: a fundamental study with benzodiazepines

Published:2018-05-01 Issue:10 Volume:10 Page:769-781
ISSN:1757-6180
Container-title:Bioanalysis
language:en
Short-container-title:Bioanalysis

Author:

Vårdal Linda¹,Øiestad Elisabeth L¹²,Gjelstad Astrid¹,Pedersen-Bjergaard Stig¹³

Affiliation:

1. Department of Pharmaceutical Chemistry, School of Pharmacy, University of Oslo, P.O. Box 1068, Blindern, 0316 Oslo, Norway

2. Department of Forensic Sciences, Oslo University Hospital, P.O. Box 4950, Nydalen, N-0424 Oslo, Norway

3. Department of Pharmacy, School of Pharmaceutical Sciences, Faculty of Health & Medical Sciences, University of Copenhagen, Universitetsparken 2, 2100 Copenhagen, Denmark

Abstract

Aim: Electromembrane extraction (EME) of weakly basic benzodiazepines was investigated (-1.47 < pKa < 5.01). Materials & Methods: 96-well EME was performed with strongly acidic conditions in the acceptor solution using 250-mM trifluoroacetic acid to maximize ionization. Results & Conclusion: Recoveries more than 80% were obtained for analytes with pKa > 2, whereas EME was less efficient for substances with pKa < 2. The latter was trapped in the supported liquid membrane due to less acidic pH conditions in the acceptor solution close to the supported liquid membrane. EME followed by UHPLC–MS/MS analysis was evaluated from human plasma, and the results were in compliance with EMA guidelines. Both electrokinetic migration and passive diffusion contributed to mass transfer when performing EME of weakly basic analytes.

Publisher

Future Science Ltd

Subject

Medical Laboratory Technology,Clinical Biochemistry,General Pharmacology, Toxicology and Pharmaceutics,General Medicine,Analytical Chemistry

Link

http://www.future-science.com/doi/pdf/10.4155/bio-2018-0030

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