Kaempferol ameliorates the regulatory effects of PVT1/miR-214 on epithelial–mesenchymal transition through the PAK4/β-catenin axis in SRA01/04 cells

Abstract

Aim: To investigate whether kaempferol exhibits a protective effect on high glucose-induced epithelial–mesenchymal transition (EMT) by mediating the  PVT1/ miR-214 and PAK4/β-catenin pathways in SRA01/04 cells. Methods & methods: qRT-PCR and western blot assays were used for gene and protein determination, and migration and invasion assays were conducted. A coimmunoprecipitation assay was used for determining protein interactions. Results: High glucose effectively upregulated PVT1 expression, downregulated miR-214 expression and promoted cell migration and invasion. Kaempferol attenuated high glucose-induced EMT by increasing PVT1 expression and decreasing miR-214 expression. PAK4 was identified as a direct target of miR-214. PAK4 overexpression could rescue the effects of PVT1 deficiency on SRA01/04 cells. Conclusion: Kaempferol ameliorated the regulatory effects of PVT1/ miR-214 on high glucose-induced EMT through PAK4/β-catenin in SRA01/04 cells.