A validated LC–MS/MS method for the quantification of piperacillin/tazobactam on dried blood spot-Reference-Cited by-同舟云学术

A validated LC–MS/MS method for the quantification of piperacillin/tazobactam on dried blood spot

Published:2014-11 Issue:21 Volume:6 Page:2795-2802
ISSN:1757-6180
Container-title:Bioanalysis
language:en
Short-container-title:Bioanalysis

Author:

Barco Sebastiano¹,Risso Francesco Maria²,Bruschettini Matteo²,Bandettini Roberto¹,Ramenghi Luca A²,Tripodi Gino¹,Castagnola Elio³,Cangemi Giuliana¹

Affiliation:

1. Clinical Pathology Laboratory Unit, Istituto Giannina Gaslini, Genoa, Italy

2. Neonatal Intensive Care Unit, Istituto Giannina Gaslini, Genoa, Italy

3. Infectious Disease Unit, Istituto Giannina Gaslini, Genoa, Italy

Abstract

Background Therapeutic drug monitoring (TDM) of antibiotics in children is of fundamental importance for effective patient management. The use of dried blood spot (DBS) offers a number of advantages over conventional blood collection. The aim of this study is to develop and validate a method to measure piperacillin/tazobactam in DBS. Results The analysis was performed by using LC–MS/MS operating in multiple reaction monitoring mode. The method has been validated by applying EMA guidelines and its suitability for TDM was evaluated by using samples from low birth weight neonates. Conclusion This paper describes a fast and cost-effective micromethod for the simultaneous determination of piperacillin/tazobactam levels on dried blood spot that is suitable for TDM in children.

Publisher

Future Science Ltd

Subject

Medical Laboratory Technology,Clinical Biochemistry,General Pharmacology, Toxicology and Pharmaceutics,General Medicine,Analytical Chemistry

Link

http://www.future-science.com/doi/pdf/10.4155/bio.14.205

Reference18 articles.

1. Dried blood spot sampling in combination with LC-MS/MS for quantitative analysis of small molecules

2. Dried Blood Spot Methods in Therapeutic Drug Monitoring: Methods, Assays, and Pitfalls

3. What a drop can do: Dried blood spots as a minimally invasive method for integrating biomarkers into population-based research

4. Dried matrix spot direct analysis: evaluating the robustness of a direct elution technique for use in quantitative bioanalysis

5. Stability of 17α-Hydroxyprogesterone in Dried Blood Spots after Autoclaving and Prolonged Storage

Cited by 14 articles. 订阅此论文施引文献订阅此论文施引文献，注册后可以免费订阅5篇论文的施引文献，订阅后可以查看论文全部施引文献

1. ADAPTATION OF “DRIED BLOOD DROP” METHOD FOR THERAPEUTIC DRUG MONITORING;Pharmacy & Pharmacology;2022-10-29

2. Development and validation of an assay for the measurement of gentamicin concentrations in dried blood spots using UHPLC-MS/MS;Journal of Pharmaceutical and Biomedical Analysis;2022-01

3. Green and sustainable drug analysis – Combining microsampling and microextraction of drugs of abuse;Sustainable Chemistry and Pharmacy;2021-12

4. A new HPLC‐MS/MS analytical method for quantification of tazobactam, piperacillin, and meropenem in human plasma;Journal of Separation Science;2021-06-15

5. Therapeutic Drug Monitoring Is a Feasible Tool to Personalize Drug Administration in Neonates Using New Techniques: An Overview on the Pharmacokinetics and Pharmacodynamics in Neonatal Age;International Journal of Molecular Sciences;2020-08-17