Capillary microsampling of whole blood for mouse PK studies: an easy route to serial blood sampling-Reference-Cited by-同舟云学术

Capillary microsampling of whole blood for mouse PK studies: an easy route to serial blood sampling

Published:2015-03 Issue:4 Volume:7 Page:449-461
ISSN:1757-6180
Container-title:Bioanalysis
language:en
Short-container-title:Bioanalysis

Author:

Korfmacher Walter¹,Fitzgerald Maria¹,Luo Yongyi¹,Ho Stacy¹,Wang Jie¹,Wu Zhongtao¹,Snow Gregory²,O'Shea Thomas¹

Affiliation:

1. Sanofi, Waltham, MA 02451, USA

2. Agilux Laboratories, Worcester, MA 01605, USA

Abstract

Background: Capillary microsampling (CMS) of 8 µl of blood provides a methodology that can be utilized for serial sampling in drug discovery mouse PK studies. Results: Blood CMS sample results were compared to plasma sample results for three compounds (with expected Cb/Cp of 1 to 2) and found to be similar. In addition, for three compounds, blood CMS results were found to be equivalent to results generated with standard whole blood sampling. In a 5-day repeated dose PK study, four mice were dosed (IV) daily and sampled on both day one and day five using blood CMS procedure. Conclusion: Blood CMS using 8 µl glass capillary microsamples provides a straightforward and effective approach for mouse serial blood sampling.

Publisher

Future Science Ltd

Subject

Medical Laboratory Technology,Clinical Biochemistry,General Pharmacology, Toxicology and Pharmaceutics,General Medicine,Analytical Chemistry

Link

http://www.future-science.com/doi/pdf/10.4155/bio.14.275

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