Sol-gel approach for extracting highly versatile aspirin and its metabolites using MISPE followed by GC

Sol-gel approach for extracting highly versatile aspirin and its metabolites using MISPE followed by GC–MS/MS analysis

Published:2016-04 Issue:8 Volume:8 Page:795-805
ISSN:1757-6180
Container-title:Bioanalysis
language:en
Short-container-title:Bioanalysis

Author:

Bhatia Tejasvi¹²,Gupta Manoj Kumar¹²,Singh Pratibha¹,Chauhan Abhishek¹,Saxena Prem Narain³,Mudiam Mohana Krishna Reddy¹²⁴

Affiliation:

1. Analytical Chemistry Laboratory, Regulatory Toxicology Group, CSIR-IITR, MG Marg, Lucknow 226001, India

2. Academy of Scientific & Innovative Research (AcSIR), CSIR-IITR, MG Marg, Lucknow 226001, India

3. CIF Facility, CSIR-IITR, MG Marg, Lucknow 226001, India

4. Pesticide Toxicology Laboratory, Regulatory Toxicology Group, CSIR-IITR, M G Marg, Lucknow 226001, India

Abstract

Aim: Aspirin is known to be a salicylate drug widely used as an analgesic, antipyretic and anti-inflammatory drug. Methodology: Sol-gel based nanosized molecularly imprinted polymer (nMIP) has been synthesized for extraction of aspirin and its metabolites in urine followed by GC–MS/MS analysis. Results: Binding affinity of nMIP and nonimprinted polymer was found to be in the range of 70–95% and 29–45%, respectively. LOD and LOQ of aspirin and its metabolites were found to be in the range of 0.63–2.4 ng/ml and 2.07–7.68 ng/ml, respectively. Conclusion: The developed method was found to be applicable for routine analysis of aspirin and its metabolites in biological samples.

Publisher

Future Science Ltd

Subject

Medical Laboratory Technology,Clinical Biochemistry,General Pharmacology, Toxicology and Pharmaceutics,General Medicine,Analytical Chemistry

Link

http://www.future-science.com/doi/pdf/10.4155/bio.16.28

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