Volumetric absorptive microsampling as an effective microsampling technique for LC–MS/MS bioanalysis of biomarkers in drug discovery-Reference-Cited by-同舟云学术

Volumetric absorptive microsampling as an effective microsampling technique for LC–MS/MS bioanalysis of biomarkers in drug discovery

Published:2023-07 Issue:14 Volume:15 Page:845-859
ISSN:1757-6180
Container-title:Bioanalysis
language:en
Short-container-title:Bioanalysis

Author:

Kapadnis Unnati¹,Locuson Chuck²,Okamura Heidi²,Rienzo Gianluca De³,Cotter Cece²,Zhu Dongwei⁴,Narayanaswami Rohini⁴,Castro-Perez Jose⁵,Marathe Punit²,Yang Wen-Chu⁶

Affiliation:

1. Biogen, 225 Binney St, Cambridge, MA 02142, USA

2. Agios Pharmarceuticals, 88 Sidney St, Cambridge, MA 02139, USA

3. Mitobridge, 1030 Massachusetts Ave., Cambridge, MA 02138, USA

4. Servier Pharmaceuticals, 200 Pier 4 Blvd., Boston, MA 02210, USA

5. Sciex, 500 Old Connecticut Path, Framingham, MA 01701, USA

6. NovaBioAssays, 52 Dragon CT, Suite 3B, Woburn, MA 01801, USA

Abstract

Aim: Develop and validate a volumetric absorptive microsampling (VAMS)-based LC–MS/MS method to support the bioanalysis of amino acid and carboxylic acid biomarkers in mouse whole blood. Method: Mouse whole blood was collected using a 10 μl VAMS device. The analytes in VAMS were extracted and analyzed using an LC–MS/MS method. Results: The VAMS-based LC–MS/MS assay exhibited a linearity range of 10.0–10,000 ng/ml with acceptable precision and accuracy and consistent recovery. The analyte stability in mouse whole blood VAMS was shown for 7 days at ambient conditions and at -80°C, as well as with three freeze/thaw cycles. Conclusion: A simple and robust VAMS-based LC–MS/MS method was developed and further validated for simultaneous bioanalysis of nine biomarkers in mouse whole blood.

Publisher

Future Science Ltd

Subject

Medical Laboratory Technology,Clinical Biochemistry,General Pharmacology, Toxicology and Pharmaceutics,General Medicine,Analytical Chemistry

Link

https://www.future-science.com/doi/pdf/10.4155/bio-2023-0059

Reference16 articles.

1. Volumetric Absorptive Microsampling: A Dried Sample Collection Technique for Quantitative Bioanalysis

2. Dried Blood Spots for Global Health Diagnostics and Surveillance: Opportunities and Challenges

3. Extractability-mediated stability bias and hematocrit impact: High extraction recovery is critical to feasibility of volumetric adsorptive microsampling (VAMS) in regulated bioanalysis

4. Neonatal and carrier screening for rare diseases: how innovation challenges screening criteria worldwide

5. Implementing Dried Blood Spot Sampling for Clinical Pharmacokinetic Determinations: Considerations from the IQ Consortium Microsampling Working Group

Cited by 1 articles. 订阅此论文施引文献订阅此论文施引文献，注册后可以免费订阅5篇论文的施引文献，订阅后可以查看论文全部施引文献

1. Volumetric Absorptive Microsampling in the Analysis of Endogenous Metabolites;Metabolites;2023-09-26