Identification and subsequent removal of an interference by FAIMS in the bioanalysis of dianicline in animal plasma-Reference-Cited by-同舟云学术

Identification and subsequent removal of an interference by FAIMS in the bioanalysis of dianicline in animal plasma

Published:2011-09 Issue:18 Volume:3 Page:2119-2127
ISSN:1757-6180
Container-title:Bioanalysis
language:en
Short-container-title:Bioanalysis

Author:

Blackburn Michael A,Kapron James¹,Mackie Jennifer²,Firth John³,Harrison Mark¹,McDougall Stuart³

Affiliation:

1. Thermo Fisher Scientific, Hemel Hempstead, UK, and Ottawa, ON, Canada

2. R-BiopharmRhone Ltd, Glasgow, UK

3. Bioanalytical Services, Covance Laboratories, Willowburn Avenue, Alnwick, Northumberland, UK

Abstract

Background: The determination of pharmacokinetic parameters requires accurate and reliable bioanalytical methods. Even using highly selective MS/MS, interferences can occur. This paper describes the source of some of these interferences with an example discussed involving the problem of a ketamine interference in a plasma assay. Results: The introduction of field asymmetric waveform ion mobility spectrometry (FAIMS) removed the interference, enhanced signal-to-background and met GLP acceptance criteria. Relative to the non-FAIMS method, assay calibration characteristics were improved. The FAIMS source gave optimal performance following the introduction of a split in order to reduce the inlet flow to approximately 0.4 ml/min. Conclusion: The introduction of ion-mobility separation into a bioanalytical LC–MS/MS method can remove unexpected isobaric interferences without the need to redevelop the chromatography.

Publisher

Future Science Ltd

Subject

Medical Laboratory Technology,Clinical Biochemistry,General Pharmacology, Toxicology and Pharmaceutics,General Medicine,Analytical Chemistry

Link

http://www.future-science.com/doi/pdf/10.4155/bio.11.194

Reference19 articles.

1. Using Research Metrics to Improve Timelines: Proceedings from the 2nd Annual CTSA Clinical Research Management Workshop

2. LC-FAIMS-MS/MS for Quantification of a Peptide in Plasma and Evaluation of FAIMS Global Selectivity from Plasma Components

3. High-Field Asymmetric Waveform Ion Mobility Spectrometry for Determining the Location of In-Source Collision-Induced Dissociation in Electrospray Ionization Mass Spectrometry

4. Workshop/conference report—Quantitative bioanalytical methods validation and implementation: Best practices for chromatographic and ligand binding assays

5. US Department of Health and Human Service, US FDA, Center for Drug Evaluation and Research. Guidance for Industry: Bioanalytical Method Validation. MD, USA (2001).

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