Choline-containing phospholipids in microdissected human Alzheimer’s disease brain senile plaque versus neuropil

Author:

Gaudin Mathieu12,Panchal Maï34,Auzeil Nicolas1,Duyckaerts Charles345,Brunelle Alain6,Laprévote Olivier1,Touboul David6

Affiliation:

1. Chimie-Toxicologie Analytique et Cellulaire, EA 4463, Faculté des Sciences Pharmaceutiques et Biologiques, Université Paris Descartes, PRES Sorbonne Paris Cité, France

2. Division Métabolisme, Technologie Servier, Orléans, France

3. Laboratoire de Neuropathologie Escourolle, Hôpital de la Salpêtrière, AP-HP, Paris, France

4. Centre de recherche de l’ICM, (UPMC, INSERM UMR S 975, CNRS UMR 7225), France

5. UPMC Paris Universitas, France

6. Centre de recherche de Gif, Institut de Chimie des Substances Naturelles, CNRS, Avenue de la Terrasse, 91198 Gif-sur-Yvette, France.

Abstract

Background: Lipidomic studies related to Alzheimer’s disease have been reported on either biological fluids or large human brain samples. For a better understanding of the role of lipids, especially during the amyloid-β peptide aggregation, it is crucial to determine the composition of the senile plaque versus the surrounding tissue, that is, the neuropil. Results: A laser microdissection step was added to the analysis by UPLC–MS/MS. Despite the very low amount of sample, two phosphatidylcholines that were significantly depleted in the senile plaque were identified. Conclusion: Changes in the phospholipid content have been shown between senile plaque versus neuropil. Nano HPLC, allowing a complete lipidomic profile, should further improve the results.

Publisher

Future Science Ltd

Subject

Medical Laboratory Technology,Clinical Biochemistry,General Pharmacology, Toxicology and Pharmaceutics,General Medicine,Analytical Chemistry

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