Antibody-free workflows for protein quantification by LC–MS/MS-Reference-Cited by-同舟云学术

Antibody-free workflows for protein quantification by LC–MS/MS

Published:2015-04 Issue:6 Volume:7 Page:763-779
ISSN:1757-6180
Container-title:Bioanalysis
language:en
Short-container-title:Bioanalysis

Author:

Wilffert Daniel¹,Bischoff Rainer¹,van de Merbel Nico C¹²

Affiliation:

1. Analytical Biochemistry, Department of Pharmacy, University of Groningen, Antonius Deusinglaan 1, 9713 AV Groningen, The Netherlands

2. PRA Health Sciences, Bioanalytical Laboratory, Westerbrink 3, 9405 BJ Assen, The Netherlands

Abstract

Antibody-free approaches for quantitative LC–MS/MS-based protein bioanalysis are reviewed and critically evaluated, and compared with the more widely used immunoaffinity-based approaches. Antibody-free workflows will be divided into four groups and discussed in the following order: direct analysis of signature peptides after proteolytic digestion; enrichment of target proteins and signature peptides by fractionated protein precipitation; enrichment of target proteins and signature peptides by reversed-phase and ion-exchange solid-phase extraction; and enrichment of target proteins and signature peptides by (antibody-free) affinity-solid-phase extraction.

Publisher

Future Science Ltd

Subject

Medical Laboratory Technology,Clinical Biochemistry,General Pharmacology, Toxicology and Pharmaceutics,General Medicine,Analytical Chemistry

Link

http://www.future-science.com/doi/pdf/10.4155/bio.14.308

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