Screening of stabilizers for LC–MS/MS analysis of clevidipine and its primary metabolite in dog whole blood-Reference-Cited by-同舟云学术

Screening of stabilizers for LC–MS/MS analysis of clevidipine and its primary metabolite in dog whole blood

Published:2015-07 Issue:12 Volume:7 Page:1457-1469
ISSN:1757-6180
Container-title:Bioanalysis
language:en
Short-container-title:Bioanalysis

Author:

Cao Peng¹,Li Gao¹²,Huang Ling¹,Zhao Shouwei¹,Hu Yang¹,Qin Lixia¹,Qiu Lihui¹,Zhu Wenwen¹,Si Luqin¹²,Huang Jiangeng¹²

Affiliation:

1. Department of Pharmaceutics, School of Pharmacy, Tongji Medical College, Huazhong University of Science & Technology, Wuhan 430030, PR China

2. Hubei Engineering Research Center for Novel Drug Delivery Systems, Huazhong University of Science & Technology, Wuhan 430030, PR China

Abstract

Background: Clevidipine is an ester-containing antihypertensive agent that undergoes rapid hydrolysis in blood. A reliable stabilizer cocktail containing citric acid and ascorbic acid was established and the LC–MS/MS method was validated for simultaneous determination of clevidipine and its major metabolite in beagle dog whole blood. Results: The stabilizer could nearly completely inhibit the esterase activity. Both analytes were extracted from whole blood by toluene and detected by MS/MS in positive ESI mode. The linearity range was 0.1–100.0 ng/ml for clevidipine and 1.0–1000.0 ng/ml for the primary metabolite. Conclusion: The stabilizer cocktail was able to effectively suppress the activity of esterase in blood. The method was successfully applied to a PK study of clevidipine in beagle dogs.

Publisher

Future Science Ltd

Subject

Medical Laboratory Technology,Clinical Biochemistry,General Pharmacology, Toxicology and Pharmaceutics,General Medicine,Analytical Chemistry

Link

http://www.future-science.com/doi/pdf/10.4155/bio.15.74

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3. Clevidipine Effectively and Rapidly Controls Blood Pressure Preoperatively in Cardiac Surgery Patients: The Results of the Randomized, Placebo-Controlled Efficacy Study of Clevidipine Assessing Its Preoperative Antihypertensive Effect in Cardiac Surgery-1

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