UHPLC–MS/MS method for iohexol determination in human EDTA and lithium-heparin plasma, human urine and in goat- and pig EDTA plasma

Author:

Stevens Jasper1ORCID,Wessels Mireille A1,Roggeveld Jan1,Koster Remco A12ORCID,Dekkers Claire CJ1ORCID,van Gelder Maaike K3,Gansevoort Ron T4,Heerspink Hiddo JL1ORCID,Touw Daan J15ORCID

Affiliation:

1. Department of Clinical Pharmacy & Pharmacology, University Medical Center Groningen, University of Groningen, Groningen, The Netherlands

2. Bioanalytical Laboratory, PRA Health Sciences, Assen, The Netherlands

3. Division of Internal Medicine & Dermatology, Nephrology & Hypertension, University Medical Center Utrecht, Utrecht, The Netherlands

4. Department of Internal Medicine, University Medical Center Groningen, University of Groningen, Groningen, The Netherlands

5. Department of Pharmaceutical Analysis, Groningen Research Institute of Pharmacy, University of Groningen, Groningen, The Netherlands

Abstract

Aim: Iohexol plasma clearance is used as an indicator of kidney function in clinical and preclinical settings. To investigate the pharmacokinetic profile of iohexol, a rapid, simple method for measurement of iohexol in different matrices and species was needed. Materials & methods: Iohexol was separated on an Accucore C18 column (Thermo Fisher Scientific, CA, USA). Detection was performed on a Thermo Scientific Quantiva tandem quadrupole mass spectrometer. The method was validated according to the requirements for bioanalytical methods issued by the US FDA and European Medicines Agency. Conclusion: We developed and validated a fast and efficient analytical method, suitable for analyzing iohexol in human EDTA plasma, human lithium-heparin plasma, human urine and goat- and pig EDTA plasma, using only one calibration line prepared in human EDTA plasma.

Publisher

Future Science Ltd

Subject

Medical Laboratory Technology,Clinical Biochemistry,General Pharmacology, Toxicology and Pharmaceutics,General Medicine,Analytical Chemistry

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5. GFR measured by iohexol: the best choice from a laboratory perspective

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