Chromatography with higher pressure, smaller particles and higher temperature: a bioanalytical perspective

Author:

Varma Deepti1,Jansen Susan A,Ganti Satya1

Affiliation:

1. Temple University, Department of Chemistry, 1901 N. 13th St, Philadelphia, PA 19122, USA

Abstract

Recent years have seen widespread use of ultra-high-pressure liquid chromatography (UHPLC) in biological fluids. Most commonly the emphasis is on developing high throughput assay methods to reduce the analysis time and cost. Particle size and temperature are chromatographic parameters that can be changed to improve efficiency and obtain rapid separations. UHPLC and high-temperature liquid chromatography (HTLC) are two techniques that reduce the analysis time by decreasing the size of the column packing material and increasing the column temperature, respectively. Both of these techniques have advantages and limitations. In this article we have summarized the history, theoretical background of UHPLC and HTLC and the various advantages and limitations of sample preparation techniques and the detection systems (mass spectrometry and ultraviolet) used for the bioanalytical assays. In addition, selected bioanalytical applications of the two techniques have been reviewed and tabulated.

Publisher

Future Science Ltd

Subject

Medical Laboratory Technology,Clinical Biochemistry,General Pharmacology, Toxicology and Pharmaceutics,General Medicine,Analytical Chemistry

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