Pharmacokinetic determination and analysis of nortriptyline based on GC–MS coupled with hollow-fiber drop-to-drop solvent microextraction technique

Abstract

Aim: A simple, sensitive and robust technique of hollow-fiber drop-to-drop solvent microextraction coupled with GC–MS has been successfully developed for the detection of antidepressant drug nortriptyline in human blood and urine samples. The recoveries of the drug from the spiked samples are found to be well within the range and appropriate to support the method. Results: The LOD for the drug was obtained to be 0.007, 0.009 and 0.021 μg ml-1 in deionized water, urine and blood samples of human subjects, respectively. Linearity was obtained over the concentration range of 0.5–5.0 mg l-1 in deionized water with correlation coefficient 0.99672.