Impact of electrospray ion source platforms on matrix effect due to plasma phospholipids in the determination of rivastigmine by LC–MS/MS

Author:

Trivedi Vikas1,Upadhyay Vivek1,Yadav Manish2,Shrivastav Pranav S3,Sanyal Mallika14

Affiliation:

1. Chemistry Department, Kadi Sarva Vishwavidyalaya, Sarva Vidyalaya Campus, Sector 15/23, Gandhinagar 382015, India

2. Bioanalytical Department, Alkem Laboratories Ltd, Lower Parel, Mumbai 400013, India

3. Department of Chemistry, School of Sciences, Gujarat University, Navrangpura, Ahmedabad 380009, India

4. Chemistry Department, St Xavier's College, Navrangpura, Ahmedabad 380009, India

Abstract

Background: This study evaluates the performance of three electrospray ionization source designs to monitor the interference of plasma phospholipids for reliable estimation of rivastigmine by LC–MS/MS for method ruggedness. The variation in the area response due to matrix effects was assessed by post-column infusion, post-extraction spiking and standard-line slope methods. Results: The observed interference due to coeluting phospholipids (m/z: 524.0/184.0) at the retention time of rivastigmine was 39.5, 12.9 and 0.4% using angular spray, orthogonal spray and dual orthogonal ion source spray design, respectively. Similarly, %CV for standard line slopes was 6.9, 4.6 and 2.0, respectively. Conclusion: Z-spray source design provided better and efficient transfer of gas phase ions into the mass analyzer compared with angular and orthogonal spray. The study showed that Z-spray ion source provided minimum interference from phospholipids compared with other ion source designs.

Publisher

Future Science Ltd

Subject

Medical Laboratory Technology,Clinical Biochemistry,General Pharmacology, Toxicology and Pharmaceutics,General Medicine,Analytical Chemistry

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