Methylmalonic acid analysis using urine filter paper samples to screen for metabolic vitamin B<sub>12</sub> deficiency in older adults-Reference-Cited by-同舟云学术

Methylmalonic acid analysis using urine filter paper samples to screen for metabolic vitamin B₁₂ deficiency in older adults

Published:2022-05 Issue:9 Volume:14 Page:615-626
ISSN:1757-6180
Container-title:Bioanalysis
language:en
Short-container-title:Bioanalysis

Author:

Boutin Michel¹^ORCID,Presse Nancy²³⁴^ORCID,Allard David³,Martineau Tristan¹,Gaudreau Pierrette⁵⁶^ORCID,Auray-Blais Christiane¹^ORCID

Affiliation:

1. Division of Medical Genetics, Department of Pediatrics, Centre de Recherche – Centre Hospitalier Universitaire de Sherbrooke, Faculty of Medicine and Health Sciences, Université de Sherbrooke, Sherbrooke, QC, J1H 5N4, Canada

2. Research Center on Aging, Centre Intégré Universitaire de Santé et de Services Sociaux de l'Estrie – Centre Hospitalier Universitaire de Sherbrooke, Sherbrooke, QC, J1H 4C4, Canada

3. Department of Community Health Sciences, Faculty of Medicine and Health Sciences, Université de Sherbrooke, Sherbrooke, QC, J1H 5N4, Canada

4. Centre de Recherche, Institut Universitaire de Gériatrie de Montréal, Montréal, QC, H3W 1W4, Canada

5. Centre de Recherche du Centre Hospitalier de l'Université de Montréal, Montréal, QC, H2X 0A9, Canada

6. Département de Médecine, Faculté de Médecine, Université de Montréal, Montréal, QC, H3C 3J7, Canada

Abstract

Aim: Methylmalonic acid (MMA) analysis in urine represents a noninvasive approach to screening for vitamin B12 deficiency in older adults. A method allowing the analysis of MMA/creatinine in fasting urine collected on filter paper was developed/validated. Method: Dry urine specimens were eluted using a solution containing internal standards, filtrated and analyzed by ultra-performance LC-MS/MS. Results: The method allowed the chromatographic separation of MMA from succinic acid. Dried urine samples were stable for 86 days at room temperature. The MMA/creatinine ratios measured in urine collected on filter paper were highly correlated with values derived from the corresponding liquid specimens. Conclusion: This robust filter paper method might greatly improve the accessibility and cost–effectiveness of vitamin B12 deficiency screening in older adults.

Funder

Merck-Frosst Chair funded by La Fondation de l'Université de Sherbrooke

Quebec Network for Research on Aging

The NuAge Databank and Biobank are supported by the Fonds de recherche du Québec

Canadian Institutes of Health Research

Research funds from the laboratory of Christiane Auray-Blais

Publisher

Future Science Ltd

Subject

Medical Laboratory Technology,Clinical Biochemistry,General Pharmacology, Toxicology and Pharmaceutics,General Medicine,Analytical Chemistry

Link

https://www.future-science.com/doi/pdf/10.4155/bio-2022-0007

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