Quantitation of vistusertib by UHPLC-MS/MS in rat plasma and its application to a pharmacokinetic study-Reference-Cited by-同舟云学术

Quantitation of vistusertib by UHPLC-MS/MS in rat plasma and its application to a pharmacokinetic study

Published:2021-09 Issue:17 Volume:13 Page:1333-1341
ISSN:1757-6180
Container-title:Bioanalysis
language:en
Short-container-title:Bioanalysis

Author:

Wang Fuqi¹,Song Danni¹,Zou Fengmao¹,Zhao Honghui²,Zhao Xu²^ORCID

Affiliation:

1. School of Traditional Chinese Material Medica, Shenyang Pharmaceutical University, Shenyang, 110016, PR China

2. Faculty of Functional Food & Wine, Shenyang Pharmaceutical University, Shenyang, 110016, PR China

Abstract

Aim: The present study aimed to develop a UHPLC-MS/MS method for determination of vistusertib in biological matrix, and to describe the pharmacokinetic behavior of vistusertib in SD rats. Methodology & results: After protein precipitation with acetone and acetonitrile (1:1), the chromatographic separation was achieved on an Agilent Poroshell 120 EC-C18 column and detected with a SCIEX QTRAP 4500 mass spectrometer under positive ionization mode. The developed UHPLC-MS/MS method showed an excellent linearity within the range of 1.0–3000 ng/ml with good accuracy and precision. Vistusertib showed a rapid absorption and reached the maximum concentration of 3532.2 ± 678.0 ng/ml 20–30 min after oral administration in Sprague-Dawley rats. Conclusion: The established analytical method was fast, sensitive and robust, and successfully applied to describe the pharmacokinetic behavior of vistusertib following an oral administration in rats.

Funder

Education Department of Liaoning Province

Young and Middle Aged Teachers' Career Development Support Project of Shenyang Pharmaceutical University

Publisher

Future Science Ltd

Subject

Medical Laboratory Technology,Clinical Biochemistry,General Pharmacology, Toxicology and Pharmaceutics,General Medicine,Analytical Chemistry

Link

https://www.future-science.com/doi/pdf/10.4155/bio-2021-0152

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