Affiliation:
1. Department of Pharmaceutical Chemistry, School of Pharmacy, University of Oslo, Norway
Abstract
Aim: Water-soluble sampling materials for DBS have been introduced to solve some of the common challenges of DBS. Methodology: Carboxymethyl cellulose (CMC) as water-soluble material was evaluated for small proteins using insulin as model analyte. 15 µl of whole blood was deposited and dried on a sheet of CMC prior to dissolvation of the whole spot, matrix precipitation with acetonitrile and LC–MS/MS analysis. Results: CMC was shown to promote matrix precipitation resulting in cleaner extracts than precipitation without CMC present. The recovery of insulin from the spot was 68 ± 4%, and the spotted samples were stable for at least 1 week in room temperature. Conclusion: Water-soluble DBS showed promising performance also in analysis of small proteins.
Subject
Medical Laboratory Technology,Clinical Biochemistry,General Pharmacology, Toxicology and Pharmaceutics,General Medicine,Analytical Chemistry
Cited by
15 articles.
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