LC–MS/MS assay of ropinirole in rat biological matrices: elimination of lysoglycerophosphocholines-based matrix effect

Abstract

Aim: To adequately support PK evaluation of ropinirole in rats following intranasal administration, it was desirable to determine ropinirole concentrations in rat plasma, brain homogenate and microdialysate. Results & methodology: A robust LC–MS/MS method has been developed for the determination of ropinirole in biological samples. Liquid–liquid extraction using ethyl acetate eliminated matrix effect due to significantly reduced levels of lysoglycerophosphocholines. The assay was fully validated with dynamic ranges of 0.01–20 ng/ml for plasma and brain homogenate samples and 0.1–200 ng/ml for microdialysate samples. Conclusion: The proposed method was accurate and precise for the quantification of ropinirole in biological samples and was successfully applied to a microdialysis study of ropinirole in rats.