Robust Ki67 detection in human blood by flow cytometry for clinical studies-Reference-Cited by-同舟云学术

Robust Ki67 detection in human blood by flow cytometry for clinical studies

Published:2016-12 Issue:23 Volume:8 Page:2399-2413
ISSN:1757-6180
Container-title:Bioanalysis
language:en
Short-container-title:Bioanalysis

Author:

Sun Yongliang¹,Yang Katherine¹,Bridal Terry²,Ehrhardt Anka G¹

Affiliation:

1. Clinical Cytometry, Clinical Translational Technologies & Operations, Bristol-Myers Squibb Co., 311 Pennington-Rocky Hill Road, Pennington, NJ 08534, USA

2. Aerotek Scientific Resources, 371 Hoes Lane, Piscataway, NJ 08854, USA

Abstract

Aim: Ki67 is a prognostic and/or predictive biomarker in patients with malignancies. Flow cytometry is a powerful technology for single-cell multiparameter analysis. Results: We developed and validated a multicolor quantitative flow cytometry assay for detection of intracellular Ki67 expression in various immune cell subsets from human blood. The assay was optimized and showed excellent precisions. Assessment of the sample stability indicated that percentage changes from the fresh sample for the reportable results of interest were within 20%, up to 72 h after blood collection in the Cyto-Chex® BCT tube. Conclusion: The validated assay is sufficiently robust to analyze clinical samples. Easy access to peripheral blood enables continuous monitoring of Ki67 expression in blood as a biomarker, for example, for immunotherapy studies.

Publisher

Future Science Ltd

Subject

Medical Laboratory Technology,Clinical Biochemistry,General Pharmacology, Toxicology and Pharmaceutics,General Medicine,Analytical Chemistry

Link

http://www.future-science.com/doi/pdf/10.4155/bio-2016-0194

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