Rapid quantification of free and glucuronidated THCCOOH in urine using coated well plates and LC–MS/MS analysis

Author:

Hädener Marianne1,Weinmann Wolfgang1,van Staveren Dave R2,König Stefan1

Affiliation:

1. Institute of Forensic Medicine, University of Bern, Bühlstrasse 20, 3012 Bern, Switzerland

2. Tecan Schweiz AG, Seestrasse 103, 8708 Männedorf, Switzerland

Abstract

Aim: Generally, urine drug testing for cannabis abuse involves measuring total concentrations of 11-nor-9-carboxy-Δ9-tetrahydrocannabinol (THCCOOH) obtained by enzymatic and/or alkaline hydrolysis of THCCOOH-glucuronide. As hydrolysis can be inconsistent and incomplete, direct measurement of the two metabolites is preferable. Methodology & results: We developed a high-throughput LC–MS/MS method for simultaneous quantification of free and glucuronidated THCCOOH in urine using coated 96-well plates for analyte extraction and column-switching chromatography. Excellent separation of the two analytes was achieved within 2.5 min, with linear ranges from 5 to 2000 μg/l for THCCOOH and from 10 to 4000 μg/l for THCCOOH-glucuronide. Conclusion: The method was successfully validated and applied to authentic urine samples from cannabis consumers, demonstrating its applicability for routine cannabinoid testing.

Publisher

Future Science Ltd

Subject

Medical Laboratory Technology,Clinical Biochemistry,General Pharmacology, Toxicology and Pharmaceutics,General Medicine,Analytical Chemistry

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