Comparison of HbA1c detection in whole blood and dried blood spots using an automated ion-exchange HPLC system

Author:

Maleska Aleksandra12,Hirtz Christophe1,Casteleyn Elise1,Villard Orianne3,Ducos Jacques2,Avignon Antoine3,Sultan Ariane3,Lehmann Sylvain1

Affiliation:

1. Université de Montpellier, CHU de Montpellier, Laboratoire de Biochimie Protéomique Clinique, INSERM U1183, F-34000 France

2. CHU Montpellier, Laboratoire de Virologie, Hôpital Lapeyronie, Montpellier, F-34000 France; INSERM U1058, Montpellier, F-34000 France

3. Endocrinology–Diabetology–Nutrition Department, University Hospital, PhyMedExp, University of Montpellier, INSERM U1046, CNRS UMR 9214, Montpellier, France

Abstract

Aim: Hemoglobin A1c (HbA1c) is a widely recognized analyte for diagnosing and monitoring diabetes. Dried blood spot (DBS) constitutes a useful alternative to blood collection by venipuncture. Analytical and clinical validation of DBS use is, however, necessary before implementation. Results/methodology: HbA1c levels from whole blood or DBS from a cohort patients with diabetes were compared. DBS specimens were stable at ambient temperature. HbA1c detection on DBS was accurate, robust, and the correlation and agreement with whole blood values was excellent. Conclusion: This study provides for the first time a complete method comparison and validation under the ISO15189 guideline using an automated HPLC system. This approach constitutes, therefore, a useful tool for diagnosing diabetes.

Publisher

Future Science Ltd

Subject

Medical Laboratory Technology,Clinical Biochemistry,General Pharmacology, Toxicology and Pharmaceutics,General Medicine,Analytical Chemistry

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