Affiliation:
1. Department of Drug Metabolism & Pharmacokinetics, Celgene Corporation, 10300 Campus Point Dr., San Diego, CA 92121, USA
Abstract
Aim: Increasing numbers of compounds requiring stability data means highly optimized methods capable of rapid turnaround are desirable during early discovery. Materials and methods/results: An advanced, generic analytical workflow for metabolic stability has been developed that utilizing ballistic gradient LC (sub 1 min run times), exact mass TOF-MS (Waters Xevo-G2-XS Q-TOF) and automated data processing (Waters UNIFI software) allowed for rapid integration and interpretation of all data produced, eliminating the need for method development and manual processing. We can analyze and process 96 compounds across two species in quadruplicate in a 24-h period with no method development. Conclusion: An advanced bioanalytical workflow has increased our capacity threefold and reduced our instrument/processing needs threefold.
Subject
Medical Laboratory Technology,Clinical Biochemistry,General Pharmacology, Toxicology and Pharmaceutics,General Medicine,Analytical Chemistry
Cited by
5 articles.
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