SPE–MS analysis of absorption, distribution, metabolism and excretion assays: a tool to increase throughput and steamline workflow

Abstract

In an effort to create faster and more efficient bioanalytical methods for drug development, many investigators have evaluated a variety of SPE–MS systems. Over the past 15 years online systems have evolved from run times of >1.5 min/sample to <10 s/sample. High-throughput SPE–MS methods for in vitro absorption, distribution, metabolism and excretion screening assays have been described by several laboratories and shown to produce results comparable to conventional LC–MS/MS systems. While quantitative analysis of small molecules in biological matrixes holds many challenges, for several applications SPE–MS methods have achieved comparable results to LC–MS/MS with the benefit of 10–30-times the throughput. Based on its distinct advantages of throughput and streamlined workflow efficiencies, SPE–MS is a useful tool for the analysis of many in vitro absorption, distribution, metabolism and excretion assays and in vivo bioanalytical studies. Further development of SPE–MS methods and analysis workflows has the potential to expand the capabilities of this technology for other challenging bioanalytical applications.